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rubicon rabbit polyclonal antibody (Proteintech)

Proteintech rubicon rabbit polyclonal antibody
Rubicon Rabbit Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rubicon rabbit polyclonal antibody/product/Proteintech
Average 92 stars, based on 1 article reviews

rubicon rabbit polyclonal antibody - by Bioz Stars, 2026-05

92/100 stars

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immunobloting rubcn (Cell Signaling Technology Inc)

Cell Signaling Technology Inc immunobloting rubcn

A Variant sparse Partial Least Squares-Discriminate Analysis (sPLS-DA) plot demonstrating group separation among ipsilateral cortical samples from wild-type sham, <t>Rubcn-mutant</t> sham, wild-type injured, and Rubcn-mutant injured mouse groups. B Volcano plots highlighting differentially expressed genes (DEGs) between injured and sham mice in wild-type (left) and Rubcn-mutant (right) samples, with red representing significantly upregulated genes and blue representing significantly downregulated genes. C Volcano plots showing DEGs between Rubcn-mutant and wild-type mice in sham (left) and injured samples (right). A data point corresponding to the Rubcn gene has been removed from the right volcano plot to minimize the y-axis. D Heatmap of gene expression across the samples demonstrating top upregulated (top-half) and downregulated (bottom-half) DEGs between Rubcn-mutant and wild-type TBI mice. E Gene Ontology Biological Process (GO:BP) analysis of significant DEGs (P-value<0.01) between Rubcn-mutant and wild-type TBI samples. Top graph shows GO-terms associated with upregulated genes (in orange) and bottom graph shows GO-terms associated with downregulated genes (in blue) in Rubcn-mutant over wild-type TBI samples with FDR<0.05. F Heat map of selected TBI-induced inflammatory DEG expression across wild-type and Rubcn-mutant sham and TBI samples. G Ingenuity Pathway Analysis (IPA) analysis comparing canonical pathways activated following TBI in wild-type versus Rubcn-mutant mice. Orange (activated) and blue (inhibited) indicate significantly enriched canonical pathways based on activation z-score, while gray dots represent pathways detected in the specified dataset that did not meet significance thresholds. H IPA analysis of lipid-related pathways activated following TBI in wild-type versus Rubcn-mutant mice. Sample size (n) were as follows: WT Sham=5, WT TBI D3=5, Rubcn Mut. Sham=4, Rubcn Mut. TBI D3=6.

Immunobloting Rubcn, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/immunobloting rubcn/product/Cell Signaling Technology Inc
Average 95 stars, based on 1 article reviews

immunobloting rubcn - by Bioz Stars, 2026-05

95/100 stars

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rabbit anti rubicon (Cell Signaling Technology Inc)

Cell Signaling Technology Inc rabbit anti rubicon

Rabbit Anti Rubicon, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti rubicon/product/Cell Signaling Technology Inc
Average 95 stars, based on 1 article reviews

rabbit anti rubicon - by Bioz Stars, 2026-05

95/100 stars

Buy from Supplier

anti rubicon (Cell Signaling Technology Inc)

Cell Signaling Technology Inc anti rubicon

Anti Rubicon, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti rubicon/product/Cell Signaling Technology Inc
Average 95 stars, based on 1 article reviews

anti rubicon - by Bioz Stars, 2026-05

95/100 stars

Buy from Supplier

rubicon (Cell Signaling Technology Inc)

Cell Signaling Technology Inc rubicon

Rubicon, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rubicon/product/Cell Signaling Technology Inc
Average 95 stars, based on 1 article reviews

rubicon - by Bioz Stars, 2026-05

95/100 stars

Buy from Supplier

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Journal: bioRxiv

Article Title: Rubicon modulates neuroimmune responses following traumatic brain injury

doi: 10.64898/2026.03.04.709622

Figure Lengend Snippet: A Variant sparse Partial Least Squares-Discriminate Analysis (sPLS-DA) plot demonstrating group separation among ipsilateral cortical samples from wild-type sham, Rubcn-mutant sham, wild-type injured, and Rubcn-mutant injured mouse groups. B Volcano plots highlighting differentially expressed genes (DEGs) between injured and sham mice in wild-type (left) and Rubcn-mutant (right) samples, with red representing significantly upregulated genes and blue representing significantly downregulated genes. C Volcano plots showing DEGs between Rubcn-mutant and wild-type mice in sham (left) and injured samples (right). A data point corresponding to the Rubcn gene has been removed from the right volcano plot to minimize the y-axis. D Heatmap of gene expression across the samples demonstrating top upregulated (top-half) and downregulated (bottom-half) DEGs between Rubcn-mutant and wild-type TBI mice. E Gene Ontology Biological Process (GO:BP) analysis of significant DEGs (P-value<0.01) between Rubcn-mutant and wild-type TBI samples. Top graph shows GO-terms associated with upregulated genes (in orange) and bottom graph shows GO-terms associated with downregulated genes (in blue) in Rubcn-mutant over wild-type TBI samples with FDR<0.05. F Heat map of selected TBI-induced inflammatory DEG expression across wild-type and Rubcn-mutant sham and TBI samples. G Ingenuity Pathway Analysis (IPA) analysis comparing canonical pathways activated following TBI in wild-type versus Rubcn-mutant mice. Orange (activated) and blue (inhibited) indicate significantly enriched canonical pathways based on activation z-score, while gray dots represent pathways detected in the specified dataset that did not meet significance thresholds. H IPA analysis of lipid-related pathways activated following TBI in wild-type versus Rubcn-mutant mice. Sample size (n) were as follows: WT Sham=5, WT TBI D3=5, Rubcn Mut. Sham=4, Rubcn Mut. TBI D3=6.

Article Snippet: The following antibodies were used for immunobloting: RUBCN (CST-8465S; 68261S), NRROS (CST; 34388S), NLRP3 (CST; 15101S), cGAS (CST; 15102S), STING (CST; 13647S), α-fodrin (BML-FG6090-0100), P62 (BD Biosciences; 610832), LC3(CST; 2775S; NB100-2220), GAPDH (CST; 2118S), HMGB1(CST; 3935S), FLAG (CST; 33542S), MYC (CST; 2276S), HIS (CST; 2365S), GFP (CST; 2955S), 4-HNE (R&D Systems; MAB3249), mouse IgG1(CST; 5415S), mouse IgG2a (CST; 61656), anti-rabbit IgG-HRP (CST; 7074S), and anti-mouse IgG-HRP (CST; 7076S) Uncropped immunoblots are presented if Fig. S5 .

Techniques: Variant Assay, Mutagenesis, Gene Expression, Expressing, Activation Assay

A Heatmap of RT-qPCR data showing expression of inflammatory genes in ipsilateral cortices of wild-type and Rubcn -mutant mice at 1 dpi (TBI D1) and 3 dpi (TBI D3) in comparison to sham. Color coding is based on z-score. B Fold change in expression levels of indicated genes in wild-type and Rubcn -mutant mice corresponding to A . C Heatmap of RT-qPCR data showing expression of inflammatory genes in ipsilateral cortices of wild-type and Rubcn -mutant mice at 29 dpi (TBI D29). D Fold change in expression levels of indicated genes in wild-type and Rubcn -mutant mice corresponding to C. Bars represent mean ± s.e.m. Sample size ( n ) for A-B were as follows: WT Sham=8, WT TBI D1=5, WT TBI D3=9, Rubcn Mut. Sham=8, Rubcn Mut. D1=7, Rubcn Mut. D3=9. Sample size ( n ) for C-D were as follows: WT Sham=6, WT TBI D29=6, Rubcn Mut. Sham=7, Rubcn Mut. TBI D29=5. Statistical analyses for all data: Two-way ANOVA with post hoc Tukey’s test and significance is represented by * P -value<0.05, ** P -value <0.005, *** P -value <0.0005, **** P -value <0.0001.

Journal: bioRxiv

Article Title: Rubicon modulates neuroimmune responses following traumatic brain injury

doi: 10.64898/2026.03.04.709622

Figure Lengend Snippet: A Heatmap of RT-qPCR data showing expression of inflammatory genes in ipsilateral cortices of wild-type and Rubcn -mutant mice at 1 dpi (TBI D1) and 3 dpi (TBI D3) in comparison to sham. Color coding is based on z-score. B Fold change in expression levels of indicated genes in wild-type and Rubcn -mutant mice corresponding to A . C Heatmap of RT-qPCR data showing expression of inflammatory genes in ipsilateral cortices of wild-type and Rubcn -mutant mice at 29 dpi (TBI D29). D Fold change in expression levels of indicated genes in wild-type and Rubcn -mutant mice corresponding to C. Bars represent mean ± s.e.m. Sample size ( n ) for A-B were as follows: WT Sham=8, WT TBI D1=5, WT TBI D3=9, Rubcn Mut. Sham=8, Rubcn Mut. D1=7, Rubcn Mut. D3=9. Sample size ( n ) for C-D were as follows: WT Sham=6, WT TBI D29=6, Rubcn Mut. Sham=7, Rubcn Mut. TBI D29=5. Statistical analyses for all data: Two-way ANOVA with post hoc Tukey’s test and significance is represented by * P -value<0.05, ** P -value <0.005, *** P -value <0.0005, **** P -value <0.0001.

Techniques: Quantitative RT-PCR, Expressing, Mutagenesis, Comparison

A Number of foot faults before and after injury in wild-type and Rubcn-mutant mice by beam walk test. B Percent of one/two-foot support (left) and three/four-foot support (right) after injury in wild-type and Rubcn-mutant mice by assessed by CatWalk test. C Percentage of ipsilateral cortical damage at 29 dpi between injured wild-type and Rubcn-mutant mice. Data represent mean ± s.e.m. Sample size (n) for A were as follows: WT Sham=11, WT TBI=13, Rubcn Mut. Sham=13, Rubcn Mut. TBI=14. Sample size (n) for B were as follows: n=7-14 mice per group as indicated. Sample size (n) for C were as follows: WT TBI=5, Rubcn Mut. TBI=5. Statistical Analyses: For A-B, mixed-effects analysis with post hoc Tukey’s test; For C, Unpaired Student’s t-test. For all data, significance is represented by * P-value<0.05, ** P-value<0.005.

Journal: bioRxiv

Article Title: Rubicon modulates neuroimmune responses following traumatic brain injury

doi: 10.64898/2026.03.04.709622

Figure Lengend Snippet: A Number of foot faults before and after injury in wild-type and Rubcn-mutant mice by beam walk test. B Percent of one/two-foot support (left) and three/four-foot support (right) after injury in wild-type and Rubcn-mutant mice by assessed by CatWalk test. C Percentage of ipsilateral cortical damage at 29 dpi between injured wild-type and Rubcn-mutant mice. Data represent mean ± s.e.m. Sample size (n) for A were as follows: WT Sham=11, WT TBI=13, Rubcn Mut. Sham=13, Rubcn Mut. TBI=14. Sample size (n) for B were as follows: n=7-14 mice per group as indicated. Sample size (n) for C were as follows: WT TBI=5, Rubcn Mut. TBI=5. Statistical Analyses: For A-B, mixed-effects analysis with post hoc Tukey’s test; For C, Unpaired Student’s t-test. For all data, significance is represented by * P-value<0.05, ** P-value<0.005.

Techniques: Mutagenesis

A Immunoblot comparing damage-associated marker (α-fodrin) and autophagy markers (P62, LC3II) in ipsilateral cortices from wild-type and Rubcn-mutant mice at 3 dpi to sham. B Densitometric quantification of immunoblot in A. C Immunoblot of ipsilateral hippocampus at 1 and 3 dpi in wild-type and Rubcn-mutant mice probed with autophagy markers. D Densitometric quantification of immunoblot in C. E Immunoblot of ipsilateral hippocampus at 1 dpi (TBI D1) and 3 dpi (TBI D3) in wild-type and Rubcn-mutant mice probed with damage-associated markers. F Densitometric quantification of immunoblot in E. G Immunofluorescence (IF) images of GFP-LC3 (green) coronal sections from wild-type and Rubcn-mutant sham and injured mice at 3 dpi stained for IBA1 (in yellow) and P62 (in magenta). Scale bar represents 50 μm. H Quantification of mean fluorescence intensity of P62 (left) and percent of P62/SQSTM1+ cells within LC3+/IBA+ cells (right) from G. All bars represent mean ± s.e.m. Sample size (n) for A-B were as follows: WT Sham=6, WT TBI D3=6, Rubcn Mut. Sham=6, Rubcn Mut. D3=6. Sample size (n) for C-F were as follows: WT Sham=6, WT TBI D1=6, WT TBI D3=6, Rubcn Mut. Sham=5, Rubcn Mut. D1=5, Rubcn Mut. D3=5. Sample size (n) for G-H were as follows: WT Sham=4, WT TBI D3=4, Rubcn Mut. Sham=4, Rubcn Mut. D3=4. Statistical analyses for all data: Two-way ANOVA with post hoc Tukey’s test and significance is represented by * P-value<0.05, ** P-value<0.005, *** P-value<0.0005, **** P-value<0.0001.

Journal: bioRxiv

Article Title: Rubicon modulates neuroimmune responses following traumatic brain injury

doi: 10.64898/2026.03.04.709622

Figure Lengend Snippet: A Immunoblot comparing damage-associated marker (α-fodrin) and autophagy markers (P62, LC3II) in ipsilateral cortices from wild-type and Rubcn-mutant mice at 3 dpi to sham. B Densitometric quantification of immunoblot in A. C Immunoblot of ipsilateral hippocampus at 1 and 3 dpi in wild-type and Rubcn-mutant mice probed with autophagy markers. D Densitometric quantification of immunoblot in C. E Immunoblot of ipsilateral hippocampus at 1 dpi (TBI D1) and 3 dpi (TBI D3) in wild-type and Rubcn-mutant mice probed with damage-associated markers. F Densitometric quantification of immunoblot in E. G Immunofluorescence (IF) images of GFP-LC3 (green) coronal sections from wild-type and Rubcn-mutant sham and injured mice at 3 dpi stained for IBA1 (in yellow) and P62 (in magenta). Scale bar represents 50 μm. H Quantification of mean fluorescence intensity of P62 (left) and percent of P62/SQSTM1+ cells within LC3+/IBA+ cells (right) from G. All bars represent mean ± s.e.m. Sample size (n) for A-B were as follows: WT Sham=6, WT TBI D3=6, Rubcn Mut. Sham=6, Rubcn Mut. D3=6. Sample size (n) for C-F were as follows: WT Sham=6, WT TBI D1=6, WT TBI D3=6, Rubcn Mut. Sham=5, Rubcn Mut. D1=5, Rubcn Mut. D3=5. Sample size (n) for G-H were as follows: WT Sham=4, WT TBI D3=4, Rubcn Mut. Sham=4, Rubcn Mut. D3=4. Statistical analyses for all data: Two-way ANOVA with post hoc Tukey’s test and significance is represented by * P-value<0.05, ** P-value<0.005, *** P-value<0.0005, **** P-value<0.0001.

Techniques: Western Blot, Marker, Mutagenesis, Immunofluorescence, Staining, Fluorescence

A Immunoblot comparing lysates of ipsilateral cortices from wild-type and Rubcn-mutant mice probed with Rubicon A (RbA) antibody. B Western blot showing immunoprecipitation of Rubicon protein from brain lysates of wild-type and Rubcn-mutant mice using Rubicon A (RbA) and Rubicon B (RbA) antibodies. * represents background signal. Blot is probed with RbB antibody. C Rubicon protein (amino-acid) sequence highlighting peptides (p1-p12 in blue) used for peptide mapping of wild-type and mutant RUBCN proteins. Red circle is highlighting Met296 as a potential alternative translation initiation site. D Normalized peptide abundance across the length of RUBCN protein in wild-type and Rubcn-mutant immunoprecipitates using RbA and RbB antibodies. E Comparative enrichment analysis showing fold change of proteins enriched in wild-type over Rubcn-mutant immunoprecipitates using RbA and RbB antibodies. F Immunoprecipitation assay showing lysates of HEK293T cells transfected with indicated plasmids for 24 h and immunoprecipitated with anti-FLAG or anti-HA agarose beads. Left: Immunoblot of immunoprecipitates with the indicated antibodies. Right: Fold change of NRROS signal in EGFP-Rubicon-Flag and Nrros-Myc/His (Nrros+Rubicon1-3) co-transfected cells to EGFP-C1 and Nrros-Myc/His (Nrros ONLY) co-transfected cells. Each FLAG IP sample was normalized to corresponding HA IP sample for quantification. Sample size (n) = 3 biological replicates for EGFP-Rubicon-Flag and Nrros-Myc/His co-transfected cells. G Immunoblot of whole cell lysates used for immunoprecipitation assay in F. H Immunoprecipitation assay showing lysates of HEK293T cells transfected with indicated plasmids for 24 h and immunoprecipitated with anti-MYC or anti-IgG antibodies. Left: Immunoblot of immunoprecipitates with the indicated antibodies. Right: Fold change of RUBCN signal in EGFP-Rubicon-Flag and Nrros-Myc/His (Nrros+Rubicon1-2) co-transfected cells to EGFP-C1 and EGFP-Rubicon-Flag (Rubicon ONLY) co-transfected cells. Each MYC IP sample was normalized to corresponding IgG IP sample. Sample size (n) = 2 biological replicates for EGFP-Rubicon-Flag and Nrros-Myc/His co-transfected cells. I Immunoblot of whole cell lysates used for immunoprecipitation assay in H.

Journal: bioRxiv

Article Title: Rubicon modulates neuroimmune responses following traumatic brain injury

doi: 10.64898/2026.03.04.709622

Figure Lengend Snippet: A Immunoblot comparing lysates of ipsilateral cortices from wild-type and Rubcn-mutant mice probed with Rubicon A (RbA) antibody. B Western blot showing immunoprecipitation of Rubicon protein from brain lysates of wild-type and Rubcn-mutant mice using Rubicon A (RbA) and Rubicon B (RbA) antibodies. * represents background signal. Blot is probed with RbB antibody. C Rubicon protein (amino-acid) sequence highlighting peptides (p1-p12 in blue) used for peptide mapping of wild-type and mutant RUBCN proteins. Red circle is highlighting Met296 as a potential alternative translation initiation site. D Normalized peptide abundance across the length of RUBCN protein in wild-type and Rubcn-mutant immunoprecipitates using RbA and RbB antibodies. E Comparative enrichment analysis showing fold change of proteins enriched in wild-type over Rubcn-mutant immunoprecipitates using RbA and RbB antibodies. F Immunoprecipitation assay showing lysates of HEK293T cells transfected with indicated plasmids for 24 h and immunoprecipitated with anti-FLAG or anti-HA agarose beads. Left: Immunoblot of immunoprecipitates with the indicated antibodies. Right: Fold change of NRROS signal in EGFP-Rubicon-Flag and Nrros-Myc/His (Nrros+Rubicon1-3) co-transfected cells to EGFP-C1 and Nrros-Myc/His (Nrros ONLY) co-transfected cells. Each FLAG IP sample was normalized to corresponding HA IP sample for quantification. Sample size (n) = 3 biological replicates for EGFP-Rubicon-Flag and Nrros-Myc/His co-transfected cells. G Immunoblot of whole cell lysates used for immunoprecipitation assay in F. H Immunoprecipitation assay showing lysates of HEK293T cells transfected with indicated plasmids for 24 h and immunoprecipitated with anti-MYC or anti-IgG antibodies. Left: Immunoblot of immunoprecipitates with the indicated antibodies. Right: Fold change of RUBCN signal in EGFP-Rubicon-Flag and Nrros-Myc/His (Nrros+Rubicon1-2) co-transfected cells to EGFP-C1 and EGFP-Rubicon-Flag (Rubicon ONLY) co-transfected cells. Each MYC IP sample was normalized to corresponding IgG IP sample. Sample size (n) = 2 biological replicates for EGFP-Rubicon-Flag and Nrros-Myc/His co-transfected cells. I Immunoblot of whole cell lysates used for immunoprecipitation assay in H.

Techniques: Western Blot, Mutagenesis, Immunoprecipitation, Sequencing, Transfection

A Immunoblot of ipsilateral cortical tissue lysates from wild-type and Rubcn-mutant mice comparing lipid peroxidation marker at 1 dpi. B Densitometric analysis of immunoblot in A. C Immunoblot of ipsilateral cortical tissue lysates from wild-type and Rubcn-mutant mice comparing lipid peroxidation marker at 3 dpi. D Densitometric analysis of immunoblot in C. Data represent mean ± s.e.m. Sample size (n) were as follows: WT Sham=6, WT TBI D1=6, WT TBI D3=6, Rubcn Mut. Sham=6, Rubcn Mut. D1=6, Rubcn Mut. D3=6.Statistical analyses: Two-way ANOVA with post hoc Tukey’s test and significance is represented by * P-value<0.05, ** P-value<0.005, *** P-value<0.0005, **** P-value<0.0001.

Journal: bioRxiv

Article Title: Rubicon modulates neuroimmune responses following traumatic brain injury

doi: 10.64898/2026.03.04.709622

Figure Lengend Snippet: A Immunoblot of ipsilateral cortical tissue lysates from wild-type and Rubcn-mutant mice comparing lipid peroxidation marker at 1 dpi. B Densitometric analysis of immunoblot in A. C Immunoblot of ipsilateral cortical tissue lysates from wild-type and Rubcn-mutant mice comparing lipid peroxidation marker at 3 dpi. D Densitometric analysis of immunoblot in C. Data represent mean ± s.e.m. Sample size (n) were as follows: WT Sham=6, WT TBI D1=6, WT TBI D3=6, Rubcn Mut. Sham=6, Rubcn Mut. D1=6, Rubcn Mut. D3=6.Statistical analyses: Two-way ANOVA with post hoc Tukey’s test and significance is represented by * P-value<0.05, ** P-value<0.005, *** P-value<0.0005, **** P-value<0.0001.

Techniques: Western Blot, Mutagenesis, Marker

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